Dual Luciferase Assay System for Rapid Assessment of Gene Expression in Saccharomyces cerevisiae
نویسندگان
چکیده
منابع مشابه
Dual luciferase assay system for rapid assessment of gene expression in Saccharomyces cerevisiae.
A new reporter system has been developed for quantifying gene expression in the yeast Saccharomyces cerevisiae. The system relies on two different reporter genes, Renilla and firefly luciferase, to evaluate regulated gene expression. The gene encoding Renilla luciferase is fused to a constitutive promoter (PGK1 or SPT15) and integrated into the yeast genome at the CAN1 locus as a control for no...
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ژورنال
عنوان ژورنال: Eukaryotic Cell
سال: 2005
ISSN: 1535-9778,1535-9786
DOI: 10.1128/ec.4.9.1539-1549.2005